Carlos Bustamante, UC Berkeley
Carlos Bustamante uses novel methods of single-molecule visualization to deepen our understanding of molecular biology. His laboratory is developing methods of single-molecule manipulation, such as optical tweezers, to characterize the elasticity of DNA, to induce the mechanical unfolding of individual protein molecules, and to investigate the machine-like behavior of molecular motors. CB is a Howard Hughes Medical Institute investigator and member of the National Academy of Sciences USA.
- Riedel C,…, Bustamante C, The heat released during catalytic turnover enhances the diffusion of an enzyme, Nature (2015)
- Liu S, … Bustamante C, A Viral Packaging Motor Varies Its DNA Rotation and Step Size to Preserve Subunit Coordination as the Capsid Fills, Cell (2014)
- Kaiser, … Bustamante C, The Ribosome Modulates Nascent Protein Folding, Science (2011)
Jonas Frisén, Karolinska Institutet
Jonas Frisén became well known early for his discoveries that nerve cells can be regenerated in the adult brain. Perhaps his foremost discovery is an innovative method for determining the age of cells in humans using carbon 14 readings made possible by atmospheric nuclear bomb testing during the cold war. JF is a member of the Nobel assembly at the Karolinska Institute.
- Ernst A, …, Frisén J, Neurogenesis in the striatum of the adult human brain. Cell (2014)
- Huttner HB, …, Frisén J, The age and genomic integrity of neurons after cortical stroke in humans. Nature Neuroscience (2014)
- Spalding KL, …, Frisén J, Retrospective birth dating of cells in humans. Cell (2005)
Thomas carell, LMU Munich
Thomas Carell is most known for his pioneering work on nucleic acid chemistry. Currently a large part of his research has been aimed to provide new insight into DNA damage and repair, DNA modifications in epigenetics and cell reprograming using molecular agents based on click chemistry.
- Pfaffeneder T, …, Carell T, Tet oxidizes thymine to 5-hydroxymethyluracil in mouse embryonic stem cell DNA, Nature Chemical Biology (2014)
- Spruijt CG, …, Carell T*, Vermeulen M*, Dynamic readers for 5-(hydroxy)methylcytosine and its oxidized derivatives, Cell (2013)
- Willibald J, …, Carell T, Click Modified Anandamide siRNA Enables Delivery and Gene Silencing in Neuronal and Immune Cells, J. Am. Chem. Soc. (2012)
Xiaowei Zhuang, Harvard University
Xiaowei Zhuang develops advanced imaging techniques, in particular single-molecule and superresolution imaging methods, to study problems of biomedical interest. Zhuang is most known for the development of the STORM superresolution microscopy technique. Since then her lab has advanced STORM resolution to the molecular scale and used STORM imaging to discover previously unknown cellular structures, such as the periodic actin-spectrin membrane skeleton in neurons. XZ is a Howard Hughes Medical Institute investigator and member of the National Academy of Sciences USA.
- Rust MJ, Bates M, Zhuang X, Sub-diffraction-limit imaging by stochastic optical reconstruction microscopy (STORM), Nature Methods (2006)
- Huang B, Wang W, Bates M, Zhuang X, Three-dimensional Super-resolution Imaging by Stochastic Optical Reconstruction Microscopy, Science (2008)
- Xu K, Zhong G, Zhuang X, Actin, spectrin, and associated proteins form a periodic cytoskeletal structure in axons, Science (2013)
Owe orwar, Karolinska Institutet
Orwar has pioneered a number of miniaturized single cell methodologies including transfection, and pharmacology tools. His research lies on the border between chemistry, physics, and biology and he is trying to develop new nanotechnology tools to better understand biological and biochemical processes, in particular membrane biophysics. Owe Orwar is a recipient of an ERC Advanced Grant and a Biotechnology entrepreneur.
- Ainla A, … Orwar O, Jesorka A. A multifunctional pipette. Lab Chip. (2012)
- Gozen I, … Orwar O, Fractal avalanche ruptures in biological membranes, Nature Materials (2010)
- Lundqvist JA, …Orwar O. Altering the biochemical state of individual cultured cells and organelles with ultramicroelectrodes. PNAS (1998)
Ulf landegren, Uppsala University
Ulf Landegren has a long tradition of developing molecular tools for high-throughput detection of DNA, RNA and proteins in biological samples. He is known primarily for pioneering several methods that are now widely used in biological research such as padlock probes and proximity ligation assays. His lab is using these and other methods in a wide range of biomedical analyses focusing on, for example malignancies, neurodegeneration, cardiovascular disease, autoimmunity and infectious disease.
- Kamali-Moghaddam M … Landegren U, Sensitive detection of Aβ oligomers in Alzheimer’s disease by proximity ligation, BMC Neuroscience (2010)
- Söderberg O, … Landegren U, Direct observation in situ of individual endogenous protein complexes. Nature Methods (2006)
- Fredriksson S, … Landegren U, Sensitive protein detection via proximity-dependent DNA ligation assays. Nature Biotechnology (2002)
Alan Rowan, University of Nijmegen
The lab of Alan Rowan is working with design and construction of supramolecular assemblies that possess catalytic, electronic, and biomimetic properties. Recently, he has developed a system of polymer hydrogels that can be used as extra-cellular matrix mimics and cell culture surfaces, leading to surprising data that challenges the current view of stiffness vs. phenotype obtained during stem cell differentiation.
- Kouwer PHJ, … Rowan AE, Responsive biomimetic networks from polyisocyanopeptide hydrogels, Nature, (2013)
- Mandal S, …Rowan AE, Therapeutic nanoworms: towards novel synthetic dendritic cells for immunotherapy, Chemical Science (2013)
- Deutman ABC, …Rowan AE, Mechanism of threading a polymer through a macrocyclic ring, Science (2008)
Sten Linnarsson, Karolinska Institutet
Sten Linnarsson is developing new technologies for single-cell sequencing and is also actively using these new methods for discovery of new cell types and classifications. His lab was recently able to use their new tools to catalog cell-types in the mouse cortex based on differences in their mRNA content.
- Zeisel A...Linnarsson S, Cell types in the mouse cortex and hippocampus revealed by single-cell RNA-seq, Science (2015)
- Islam S...Linnarsson S, Quantitative single-cell RNA-seq with unique molecular identifiers, Nature Methods (2013)
- Pihlak A ... Linnarsson S. Rapid genome sequencing with short universal tiling probes, Nature Biotech. (2008)
Fredrik Höök, Chalmers University of Technology
Höök develops surface-based bioanalytical sensors, with special emphasis on studies of cell-membrane mimics for medical diagnostic and drug-discovery applications. His laboratory is presently focused on single-molecule studies of liposomes and individual membrane proteins. FH was awarded the Göran Gustafsson prize in Physics and he is a member of The Royal Swedish Academy of Engineering Sciences (IVA).
- M. Rabe, …F. Hook, Hydrolysis of a Lipid Membrane by Single Enzyme Molecules: Accurate Determination of Kinetic Parameters. Angewandte Chemie-Int. Ed. (2015)
- Bally M, …Hook F. Interaction of Single Virus-like Particles with Vesicles Containing Glycosphingolipids. Phys. Rev. Lett. (2011)
- Simonsson S, …Hook F. Continuous Lipid Bilayers Derived from Cell Membranes for Spatial Molecular Manipulation. J. Am. Chem. Soc. (2011)
Ana Teixeira, Karolinska institutet
Ana Teixeira uses methods from polymer science and nanotechnology to understand how cells interact with their microenvironment. Her laboratory is interested in addressing the how biophysical signals regulate cellular responses in cancer, development and regeneration.
- A. Shaw, ..., AI Teixeira, Spatial control of membrane receptor function using ligand nano-calipers. Nature Methods (2014)
- JM Dias, ..., AI Teixeira, CtBPs sense microenvironmental oxygen levels to regulate neural stem cell state, Cell Reports (2014)
- A Herland, ..., AI Teixeira, Electrochemical control of growth factor presentation to steer neural stem cell differentiation. Angew. Chem. Int. Ed. (2011)